Molecular Characteristics of Extended-Spectrum β-Lactamases in Clinical Isolates from Escherichia coli at a Japanese Tertiary Hospital
نویسندگان
چکیده
The prevalence of ESBL has been increasing worldwide. In this study, we investigated the molecular characteristics of ESBL among clinical isolates of Escherichia coli from a Japanese tertiary hospital. A total of 71 consecutive and nonduplicate clinical isolates of ESBL-positive E. coli collected at Tohoku University Hospital between January 2008 and March 2011 were studied. The antimicrobial susceptibility profile of these strains was determined. PCR and sequencing were performed to identify genes for β-lactamase (bla(TEM), bla(SHV), bla(OXA-1-like), and bla(CTX-M)) and plasmid-mediated quinolone resistance determinants (PMQR). The isolates were also analyzed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Of the 71 strains, 68 were positive for CTX-M, 28 were positive for TEM, four were positive for OXA-1, and one was positive for SHV. Sequencing revealed that CTX-M-14 was the most prevalent (31/71), followed by CTX-M-27 (21/71) and then CTX-M-15 (9/71). Of the 28 TEM-positive strains, one was TEM-10 and the rest were TEM-1. One SHV-positive strain was SHV-12. The 21 CTX-M-27-producing isolates were divided into 14 unique PFGE types, while the 9 CTX-M-15 producers were divided into 8 types. Based on MLST, 9 CTX-M-14 procedures, 19 CTX-M-27 procedures, and 8 CTX-M-15 producers belonged to ST131. Thirty-five (94.6%) of the 37 ST131 E. coli strains showed resistance to levofloxacin, which was a higher rate than among non-ST131 strains (63.6%). Among ESBL-producing isolates, one, two, and six possessed qnrB, qnrS, qepA, and aac(6')-Ib-cr, respectively. Of the 6 isolates with aac(6')-Ib-cr, 4 carried the CTX-M-15 gene. Our data suggest that CTX-M-15-producing E. coli ST131 has emerged as a worldwide pandemic clone, while CTX-M-27 (a variant of CTX-M-14) is also spreading among E. coli ST131 in Japan.
منابع مشابه
Phylogenetic typing and detection of extended-spectrum β-lactamases in Escherichia coli isolates from broiler chickens in Ahvaz, Iran
This study was conducted to reveal the phylogenetic background, to detect the genes encoding TEM, SHV and CTX-M-15 extended-spectrum β-lactamases (ESBL), and to analyze their distribution in phylo-groups of 150 Escherichia coli isolates from broiler chickens in Ahvaz (Southwest of Iran). Seventy- five cloacal swabs from healthy birds (fecal isolates), and 75 heart blood samples from bi...
متن کاملThe multidrug resistance and production of extended-spectrum beta-lactamases ctx-m, per and ver in Escherichia coli isolates derived from raw dairy samples
The occurrence of extended-spectrum beta-lactamases-producing bacteria is an important public health issue. The aim of this study was to investigate phenotypic and genotypic characteristics regarding the presence of extended spectrum β-lactamase ctx-m, per and ver producing Escherichia coli isolated from raw dairy samples. For this purpose, E. coli were isolated from 247 raw dairy samples (milk...
متن کاملآنالیز اینتگرونها و کاستهای ژنی مربوطه در ایزولههای بالینی اشریشیاکلی
Background and Objective: Horizontal transfer of integrons is the most successful transfer of antimicrobial resistance genes and the emergence of multi-drug resistance strains. The aim of the present study was to investigate the prevalence of class I and II integrons and their gene cassette assortments and antibiotic resistance profile in Escherichia coli strains isolated from diarrheal and uri...
متن کاملStudy of Antibiotic Susceptibility Pattern and Frequency of Extended Spectrum β-lactamases Genes TEM and SHV in Urinary Tract Isolates of Escherichia coli and Klebsiella pneumoniae in Tabriz
Background & objectives: Urinary tract infection (UTI) is one of the most common types of human infections and Escherichia coli and Klebsiella pneumonia are the main causes of urinary tract infection among the gram negative bacteria. The prevalence of extended-spectrum β-lactamases (ESBLs) among these bacteria and hence resistant strains to β-lactam antibiotics have increased in recent decades...
متن کاملA comparative study of the prevalence of extended-spectrum β-lactamaseproducing Escherichia coli among urinary and fecal Escherichia coli isolates of women with urinary tract infection in Kerman city
Background & Aim: Important challenge for treatment of urinary tract infections is the spread of extended-spectrum beta-lactamases (ESBLs) producing Escherichia coli. Therefore, the frequencies of main beta-lactamase genes, resistance to beta-lactams and the ability of ESBLs production in urinary and fecal Escherichia coli of women with urinary tract infections were compared. Methods: In 60 Es...
متن کاملqnr Prevalence in Extended Spectrum Beta-lactamases (ESBLs) and None- ESBLs Producing Escherichia coli Isolated from Urinary Tract Infections in Central of Iran
Objective(s) Extensive use of quinolones has been associated with raising level of resistance. In the current, we focused on assessing the prevalence of Escherichia coli resistance to quinolones and frequency of qnrA, qnrB and qnrS in non ESBLs (extended spectrum beta-lactamases) and ESBLs producing E. coli with blaSHV and blaTEM. Materials and Methods One hundred and fifty E. coli isolates ...
متن کامل